Figure 6

Effects of naringin on the mRNA expression of Sema3a, β-catenin and Nrp1.
Four weeks following USN, the total RNA was collected from the tibias ipsilateral to USN in the different treatment groups and subjected to qRT-PCR. Sema3a (a), β-catenin (b) and Nrp1 (c) in the tibias ipsilateral to USN in each group were analysed by qRT-PCR corrected by β-Actin relative to the Sham group and reverse transcribed (d). ap < 0.05, aap < 0.01, aaap < 0.001 vs. Sham group. bp < 0.05, bbp < 0.01, bbbp < 0.001 vs. USN group. cp < 0.05, ccp < 0.01, cccp < 0.001 vs. 300 mg/kg Naringin group. L represents the 30 mg/kg Naringin group, M the 100 mg/kg Naringin group and H the 300 mg/kg Naringin group. One-way ANOVA. Full-length gels are presented in Supplementary Figure S1.