Figure 5

CLSM images of cellular uptake and distribution of TMV₄, TMV₈ and TMV₁₇ in (a) HeLa cells and (b) HUVEC cells after co-incubation for 4 hours with TMV.

Green color collected from 500–550 nm show F-actin stained by Alexa Fluore-488 phalloidin. Cell nucleus were stained by To-pro-3 (collected from 662–737 nm) and the color was changed to blue by the CLSM software. RB was shown in red color (collected from 570–610 nm), indicating the distribution of TMV rods. The scale bars in figures represent 20 μm.