Figure 3 | Scientific Reports

Figure 3

From: Disorder prediction-based construct optimization improves activity and catalytic efficiency of Bacillus naganoensis pullulanase

Figure 3

SDS-PAGE analysis of (a) the cell-free extracts and (b) the purified recombinant enzymes. For cell-free extracts, lane 1–9 meant the total soluble protein of the construct PUL, PULΔN5, PULΔN22, PULΔN45, PULΔN64, PULΔN78, PULΔN106, PULΔC9 and PULΔC36, respectively. For purified enzymes, lane 1–9 meant the purified PUL, PULΔN5, PULΔN22, PULΔN45, PULΔN78, PULΔN64, PULΔN106, PULΔC9 and PULΔC36, respectively. Lane M meant the protein molecular weight marker. The amount of loaded sample was 10 μL in each lane.

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