Figure 4
Effect of TAK165 and ATRA on RARα activation.
(a,b) RARB and PXN mRNA levels in HL60, NB4 and HL60R cells as determined by real-time PCR. The cells were treated with TAK165 (100 nM for HL60 and HL60R, 50 nM for NB4) and ATRA (1 μM for HL60 and HL60R, 2 nM for NB4) for 1 day. GAPDH expression was used as an internal control gene. (c) CD11b expression in the HL60R cells. The cells were treated withTAK165 (100 nM) in the presence of vehicle or 1 μM ATRA for 3 day. A 10 μM ATRA treatment was used to indicate the resistance of the HL60R cells to ATRA. (d) Western blot analysis of RARα in the HL60R cells transduced with the different lentivirus. (e) CEBPB, RARB and PXN mRNA levels in the HL60R-pCCL and HL60R-RARα cells as determined by real-time PCR. The cells were treated with TAK165 (100 nM) in the presence of vehicle or 1 μM ATRA for 1 day. (f) CD11b expression in the HL60R-pCCL and HL60R-RARα cells. The cells were treated withTAK165in the presence of vehicle or 1 μM ATRA for 3 days. In (a,b,e,f), data presented are the mean ± SD of 3 independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001.n.s, p > 0.05.
