Figure 6: On-line measurement of biomarkers secretion from primary hepatocyte bioreactor construct upon drug treatment. | Scientific Reports

Figure 6: On-line measurement of biomarkers secretion from primary hepatocyte bioreactor construct upon drug treatment.

From: Automated microfluidic platform of bead-based electrochemical immunosensor integrated with bioreactor for continual monitoring of cell secreted biomarkers

Figure 6

(a) Photograph of the fabricated bioreactor with bioprinted primary hepatocyte spheroids. (b,c) Continual EC measurements of TF (b) and ALB (c) production rate in primary hepatocyte bioreactor with 5 mM and 10 mM APAP treatment. (d) Live/dead staining and cell viability quantification of primary hepatocyte spheroids treated with 5 mM, and 10 mM APAP. Staining was performed at Day 1, 3, and 5 using calcin AM (green) and ethidium homodimer (red). Data are representative of three independent experiments (ns = not significant; *P < 0.05; **P < 0.01; ***P < 0.001).

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