Figure 2 | Scientific Reports

Figure 2

From: Twist-mediated Epithelial-mesenchymal Transition Promotes Breast Tumor Cell Invasion via Inhibition of Hippo Pathway

Figure 2

Overexpression of Snail or Twist induces EMT in T47D and MCF7 cells.

(A) Representative images show expression of E-cadherin and ERα in Snail- or Twist-expressing T47D and MCF7 cells analyzed by immunofluorescent staining. Nuclei were visualized with DAPI staining (blue). The morphologic changes associated with EMT are shown in representative phase contrast images. Scale bars, 50 μm. (B) Expression of E-cadherin, N-cadherin and ERα in these cells was assessed by western blot analysis; actin served as a loading control. (C) Quantification of the relative mRNA levels of E-cadherin and ERα in Twist expressing T47D cells compared with vector-control cells using real-time PCR. Presented data are the mean ± SD from three separate experiments, with *and **indicate p < 0.01 in comparison with that of control.

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