Figure 1
PHD1 deficiency induces glucose intolerance and insulin resistance in standard chow-fed mice.
Body weight (A) and weights of liver, epididymal white adipose tissue (eWAT) and subcutaneous white adipose tissue (scWAT) were measured at sacrifice in 12 week-old WT (open bars) and PHD1−/− (black bars) mice on standard chow diet (B). Daily food intake (C) was monitored during 5 weeks. Fasting plasma triglycerides (D), total cholesterol (E), glucose (F) and insulin (G) levels were determined in 6-hour unfed mice and HOMA-IR (H) was calculated. An intraperitoneal glucose tolerance test (ipGTT, 2 g/kg total body weight) was performed in 6-hour unfed mice. Blood glucose levels were measured at the indicated time-points (I) and the AUC of the glucose excursion curve was calculated as a surrogate for whole-body glucose tolerance (J). The plasma insulin level during ipGTT was measured at 15 minutes (K). An intraperitoneal insulin tolerance test (0.5 U/kg total body weight) was performed in 4-hour unfed mice. Blood glucose levels were measured at the indicated time-points (L) and the AUC of the glucose excursion curve was calculated as a surrogate for whole-body insulin sensitivity (M). Data are means ± SEM (n = 7 for WT; n = 13 for PHD1−/−). #p < 0.05 vs WT mice.
