Figure 2

PHD1 deficiency promotes weight gain and insulin resistance but does not worsen high fat diet-induced metabolic alterations.

WT (open bars) and PHD1−/− (black bars) mice were fed a low-fat (LFD, 10% fat) or high-fat (HFD, 45% fat) diet for 12 weeks. Body weight was monitored throughout the experimental period (A). Delta (Δ) change in body weight from the start of diet (B), weight of liver, epididymal (eWAT) and subcutaneous (scWAT) white adipose tissue and skeletal (Sk.) muscle (C) were measured after sacrifice at week 12. Mean daily food intake (D) was recorded during 12 weeks. At week 12, plasma triglycerides (E), total cholesterol (F), glucose (G) and insulin levels (H) were measured in 6-hour unfed mice and HOMA-IR (I) was calculated. An intraperitoneal GTT (2 g/kg of total body weight) was performed in 6-hour unfed mice at week 11. Blood glucose levels were measured at the indicated time-points (J) and the area under the curve (AUC) of the glucose excursion curve was calculated as a measure of glucose tolerance (K). The plasma insulin level during ipGTT was measured at 15 minutes (L). Data are means ± SEM (n = 4 for LFD-WT; n = 7 for LFD-PHD1−/−; n = 5 for HFD-WT; n = 7 for HFD-PHD1−/−). *p < 0.05 vs LFD-fed mice, ^#p < 0.05 vs WT mice.