Figure 1 | Scientific Reports

Figure 1

From: Erythroleukemia cells acquire an alternative mitophagy capability

Figure 1

Generation of Atg7 knockout K562 cell line with CRISPR/Cas9.

(A) Schematic diagram of Atg7 gene coding region and the targeting loci of gRNA: CRISPR/Cas9. (B) DNA electrophoresis of Atg7 PCR products in wild-type and Atg7−/− K562 cells. (C) The DNA sequencing results of wild-type and Atg7−/− K562 cells. (D) Detection of ATG7, ATG5-ATG12 conjugation and the conversion of LC3-I to LC3-II by immunoblotting.

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