Figure 3
From: Erythroleukemia cells acquire an alternative mitophagy capability
Mitophagy remains functional when ATG7-dependent autophagy is defective.
(A) Histogram showing the mitochondrial mass by flow cytometry in wild-type and Atg7−/− cells. (B) A graph showing the ROS level by flow cytometry in wild-type and Atg7−/− cells. (C) Representative macroautophagy in wild-type and Atg7−/− cells. (D) Quantification analysis of the mitochondria by flow cytometry in wild-type and Atg7−/− cells treated with 20 μM CCCP and 10 nM Baf-A1. (E) Detection of the expression mitochondrial protein TOMM20, ATG7 and the conversion of LC3-I to LC3-II in K562 cells treated by 20 μM CCCP and 10 nM Baf-A1 by immunoblotting. (F) Detection of the expression of key alternative autophagy related proteins (RAB9A, BECN1, ULK1 and VPS34) by immunoblotting in K562 cells treated with 20 μm Etoposide for 18 h. (G) Detection of the expression mitochondrial protein TOMM20, ATG7 and the conversion of LC3-I to LC3-II in wild-type and Atg7−/− K562 cells treated by 20 μM CCCP and 0.1 μg/mL BFA by immunoblotting. (H) The mitochondrial mass analysis in wild-type and Atg7−/− K562 cells treated by 20 μM CCCP and 0.1 μg/mL BFA.
