Figure 4
From: Erythroleukemia cells acquire an alternative mitophagy capability
The alternative mitophagy is RAB9A-dependent.
(A) Detection of the mRNA level of Rab9A by quantitative PCR in K562 cells. (B) Western blot analysis of RAB9A. (C) The colocalization of RAB9A and mitochondria in wild-type and Atg7−/− K562 cells treated with CCCP. The nuclei, RAB9A and mitochondria were respectively stained with DAPI (blue), Dylight 488 (green) and Deep Red (red). (D) The mitochondrial mass analysis in wild-type and Atg7−/− K562 ells treated with CCCP and Baf-A1 when Rab9A was knocked down. (E) The mitochondrial mass analysis in wild-type and Atg7−/− K562 cells treated with CCCP and BFA when Rab9A was knocked down. (F) Detection of the expression of mitochondrial protein TOMM20 by immunoblotting in wild-type and Atg7−/− K562 cells treated with CCCP and BFA when Rab9A was knocked down.
