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Figure 1

From: Reverse Transcription Cross-Priming Amplification–Nucleic Acid Test Strip for Rapid Detection of Porcine Epidemic Diarrhea Virus

Figure 1

CPA-NATS and gel electrophoresis results of different components and conditions optimization: (a) external primers, (b) probe, (c) cross primer, (d) dNTP, (e) MgSO4, (f) betaine, (g) Bst DNA polymerase, (h) reaction time of 60 min, (i) reaction time of 75 min, (j) reaction time of 90 min and (k) agarose gel electrophoresis confirmations of the CPA reaction corresponding to the results on the strips. N, negative control.

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