Figure 2 | Scientific Reports

Figure 2

From: Reverse Transcription Cross-Priming Amplification–Nucleic Acid Test Strip for Rapid Detection of Porcine Epidemic Diarrhea Virus

Figure 2

Sensitivity and specificity of RT CPA-NATS and RT-PCR: (a) sensitivity of CPA-NATS. N means negative control. −5, −6, −7 means 10−5, 10−6, 10−7 dilution. The detection limit was defined to 10−6 dilution, because 10−7 dilution plasmid was detected out randomly. (b) specificity of RT CPA-NATS. N: Negative control. The pathogens used to detect the specificity of RT-NATS and RT-PCR were CSFV, PRRSV, PCV, TGEV, PRV and PEDV. (c) sensitivity of RT-PCR. M: DNA Ladder DL2000. Lane 1 and 2 is negative control. Line 3–8 is 10−1~10−6. The detection limit was 10−6 dilution. The products size is about 150 bp. (d) gel electrophoresis confirmation of RT CPA-NATS. M: DNA Ladder DL2000. Lane 1: Negative control. Lane 2~Lane 8: PEDV, CSFV, PRRSV, PCV, TGEV and PRV.

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