Table 1 Primers and probes used in CPA-NATS of PEDV.

From: Reverse Transcription Cross-Priming Amplification–Nucleic Acid Test Strip for Rapid Detection of Porcine Epidemic Diarrhea Virus

 

Primers name

Primers Sequence(5′-3′)

Labeling terminal and

Primers amplified N gene

N-F

ATGGCTTCTGTCAGCTTTCAGG

 

N-R

TTAATTTCCTGTGTCGAA

 

Outer primers

PEDVNgeneF3-1

GGCAACAACAGGTCCAGAT

 

PEDVNgeneB3-1

CGGTCATTTGACTGGTTCC

 

Probes

PEDVNgeneDF5F-12

TCAGAACAGAGGAGGCAATA

5′FAM

PEDVNgeneDF5B1

GAGTAACAACAGAGGCAACAAC

5′BIOTIN

Cross primer

PEDVNgeneCPR-2

AGAGGCAACAACCAGTCC-TTGGACTGGTTACGAGACT

 

Detection primer of PEDV

D-800-F

GCTGTGAGTAATCCGAGTT

 

D-800-R

GCTTAGGCTTCTGCTGTT

 
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