Figure 1: NOTCH signaling is activated in human HNSCC.

(A) Over-expression of NOTCH1, HES1 inhuman HNSCC (right panel) as compared with normal oral mucosa (OM, left panel) and mucosal dysplasia (Dys); scale bars, 50 μm. (B) Histoscore of immunohistochemical staining of NOTCH1 (left panel) and HES1 (right panel). Each sample score present as a dot, One way ANOVA followed by Tukey test by Graph Pad Prism 5, *P < 0.05; **P < 0.01. (C) Hierarchical clustering of NOTCH1, HES1 with CD44, SOX2, ALDH1 and Slug. Immunohistochemical staining was clustered with Cluster and visualized with Java Treeview.