Figure 4: NOTCH1 inhibition reduces stemloid cancer cell in CAL27 xenograft model. | Scientific Reports

Figure 4: NOTCH1 inhibition reduces stemloid cancer cell in CAL27 xenograft model.

From: NOTCH1 inhibition enhances the efficacy of conventional chemotherapeutic agents by targeting head neck cancer stem cell

Figure 4

(A) Schematic diagram for the CAL27 xenograft implantation and drug delivery. NOTCH signaling inhibitor DAPT (10 mg/kg, 20 mg/kg, respectively) or equivalent volume 5% ethanol PBS (vehicle) was given by intraperitoneal injection (i.p) once a day (q.d) in CAL27 cells xenograft nude mice for consecutively two weeks (n = 10 mice, respectively). (B) Representative images showed tumor regression in HNSCC xenograft treated with DAPT (right) as compared with control group. Dahs lines were utilized to depict the outline of tumor lump. Scale bar, 1 cM. (C) 10 mg/kg and 20 mg/kg DAPT treatment significant delay tumor growth; mean ± SEM, *P < 0.05; **P < 0.01; ***P < 0.001. (D) Tumor lump dissected from xenograft mice at 28 days after euthanasia; scale bar, 1 cm. (E) Box and whiskers plot revealed the weight changes of DAPT- or vehicle-treated mice; *P < 0.05, one-way ANOVA. (F) Western blotting analysis indicated DAPT treatment decreased cancer stem cells related markers ALDH1, BMI1 and SOX2 as compared with control group. GAPDH was used as loading control. (G) Representative photo of mice xenograft showed DAPT reduced CD44 and ALDH1 immunoreactivity as compared with control group; Scale bar, 50 μm.

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