Figure 6: NOTCH inhibition reduces chemoresistance of CAL27 cell xenograft. | Scientific Reports

Figure 6: NOTCH inhibition reduces chemoresistance of CAL27 cell xenograft.

From: NOTCH1 inhibition enhances the efficacy of conventional chemotherapeutic agents by targeting head neck cancer stem cell

Figure 6

(A) Tumor growth curve and (B) representative photo of DTX, CDDP, 5-FU, DAPT or combinational chemotherapy. 10 mg/kg DTX or CDDP were infused once at day 18 and 15 mg/kg 5-FU was infused for consequent 5 days from day18 to day 22. The DAPT only group receive 10 mg/kg DAPT every day and combined group receive additional 10 mg/kg DAPT every day from (as indicated by dash) from day 25 to day 35. n = 5 mice respectively. Data represents the mean ± SEM of tumor volumes from each group.*P < 0.05; ***P < 0.01. (C) DAPT effectively decreased the proportion of side population directly harvest from CAL27 xenografts tumor as compared with independent chemotherapeutical drugs. Xenograft tumor were stained with Hoechst 33342 and excited by UV light; Hoechst Red, 675 nm channel; Hoechst Blue, 450 nm channel. (D) Quantification of side population from triple experiments; *P < 0.05; **P < 0.01; ***P < 0.001. (E) DAPT effectively decreased the percentage of CD44+ CD133+ cell population in CAL27 xenograft. Digits indicated the percentage of CD44+ and CD133+ double positive cells. (E) Quantification of side population from triple experiments; **P < 0.01; ***P < 0.001.

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