Figure 2: Sequence changes following evolution of VSV-MΔ51 in MRC-5 cells.
From: Constrained evolvability of interferon suppression in an RNA virus
For each virus (founder, L1–L3), a genome map is shown with the five VSV genes (N, P, M, G, L) indicated in boxes. The leader (le) and trailer (tr) regions were also sequenced by the Sanger method. The MΔ51 virus contained a GFP gene inserted between G and L. Mutations detected by Sanger sequencing are indicated with colored vertical lines (red: non-synonymous, blue: synonymous/non-coding). The original Δ51 deletion is indicated with a yellow triangle and was preserved in all evolved lines. The scatter plots represent the frequency (0.5–100% range) of single-nucleotide polymorphisms as determined by Illumina ultra-deep sequencing. Changes detected by the Sanger method are represented as colored dots. A list of all SNPs detected by Illumina sequencing is provided in Supplementary Data S1.
