Figure 3 | Scientific Reports

Figure 3

From: Knockdown of lncRNA-ATB suppresses autocrine secretion of TGF-β2 by targeting ZNF217 via miR-200c in keloid fibroblasts

Figure 3

ZNF217 is a transcriptional activator of TGF-β2 in keloid fibroblasts.

(A) Schematic representation of ZNF217 binding site in the TGF-β2 promoter region. TSS: transcription start site; (B) ChIP assays using anti-ZNF217 antibody or human IgG as a control on the TGF-β2 promoter region, followed by PCR using ZNF217 binding site-specific primers for measurement of TGF-β2 expression; (C) qRT-PCR determination of relative ZNF217 expression levels in keloid fibroblasts (KF) treated with a control siRNA (siRNA-control) or ZNF217 specific siRNAs (siRNA-ZNF217-1 and siRNA- ZNF217-2); (D,E) qRT-PCR and ELISA determination of relative TGF-β2 mRNA and protein expression levels in KF treated with ZNF217-specific siRNAs, respectively.

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