Figure 6
Knockdown of lncRNA-ATB upregulates miR-200c expression in keloid fibroblasts.
(A) Correlation between miR-200c and ZNF217 mRNA expression in keloid tissue; (B) the predicted binding sequences of human miR-200c to the wild-type and mutant 3′-UTR of ZNF217; (C) relative luciferase activity in lncRNA-ATB-knockdown keloid fibroblasts (KF) co-transfected with plasmid containing wild-type (ZNF217-UTR-wt) or mutant 3′-UTR of ZNF217 (ZNF217-UTR-mut) and scrambled (control) or miR-200c inhibitor (inh 200c). Luciferase activity was measured 48 h after transfection; (D,E) the mRNA and protein expression of ZNF217 in stable KFs transfected with different shRNAs as assessed in the presence or absence of an miR-200c inhibitor (inh 200c); (F) luciferase activity in stable KFs transfected with luciferase reporters containing ZNF217 3′-UTR, inhibitor, or control. Data are presented as the relative ratio of firefly luciferase activity to renilla luciferase activity.
