Figure 5: MiR-19a-3p/19b-3p negatively regulates multiple players in fibrosis. | Scientific Reports

Figure 5: MiR-19a-3p/19b-3p negatively regulates multiple players in fibrosis.

From: Autophagy inhibition of hsa-miR-19a-3p/19b-3p by targeting TGF-β R II during TGF-β1-induced fibrogenesis in human cardiac fibroblasts

Figure 5

(a) Photographs of the cell invasion through the polycarbonate membrane stain by crystal violet. The migratory cell numbers of human cardiac fibroblasts transfected with miR-19a-3p/19b-3p mimics were significantly more than that of cells transfected with miR Control respectively. (b) The inhibitory effect of miR-19a-3p/19b-3p on the invasion of the cells was quantified. **P < 0.01 compared with control. Data were presented as the mean ± SEM of three separate experiments. (c,d) The Q-PCR analyses of the expression of miR-19a-3p/19b-3p in human cardiac fibroblasts transfected with miR-19a-3p/19b-3p mimics, NC and untranfected one. −△△Ct = −(Ctx-CtU6x)-(CtNCx-CtU6NC). Data were present as mean ± SEM, n = 3, **P < 0.01 vs. NC group. (e) Human cardiac fibroblasts were transfected with miR-19a-3p/19b-3p mimics, miR Control. Expression of MMP-2, MMP-9, Vimentin and α-SMA after 72 hours were detected by western blotting. Protein loading was confirmed using GAPDH. (f) Quantitative data of densitometric analyses. The ratio of MMP-2, MMP-9, Vimentin and α-SMA to GAPDH were displayed as mean ± SEM, n = 3, *p < 0.05, **p < 0.01 vs. NC group.

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