Figure 2
From: Anti-gastric cancer activity in three-dimensional tumor spheroids of bufadienolides
3D spheroid assay model of HGC-27.
(a) Morphology of HGC-27 spheroids with different cell seeding densities on day 1-day 4. Scale bar: 500 μm. (b) The diameter of spheroids with different cell seeding densities as a function of culturing time in 96-well ULA round-bottomed plates. Data represents mean ± s.d. from 2 independent measurements, each in duplicate (n = 4). (c) Reproducibility of spheroids on day 4 across 4 plates, each plate containing 60 spheroids.
