Figure 1

AtOPR3 specifically inhibited primary root growth in Arabidopsis under P deficiency.

(a) Five-day-old seedlings were transferred to full nutrient (Control), low N (-N), low P (-P), or low K (-K) conditions respectively for 7 days. WT, wild type; Atopr3, the mutant line. Results were presented as means (n = 30) with error bars (standard deviation) and asterisks indicated significant differences as determined by a t-test analysis (*P < 0.05; **P < 0.01). (b) Comparision of primary root length among WT, Atopr3 and three representative AtOPR3 complementary lines (#10, #11 and #12). Results were presented as means (n = 30) with error bars (standard deviation) and different letters indicated significant differences between different lines within the same treatment (P < 0.05). (c) The relative expression level of AtOPR3 in WT during a 7-day low P treatment. Primary roots were sampled at 0d, 1d, 3d, 5d and 7d after transfer and expression levels of AtOPR3 were determined by RT-qPCR. Data represented as means and SD (standard deviation) of three independent biological replicates. (d) Relative expression levels of six known genes regulating root response to low P in WT and Atopr3 mutant plants under the whole nutrient (Control) or low P (-P) treatment. Root samples were harvested 7d after transfer and mRNA abundance was determined by RT-qPCR. Error bars represented SD of three independent biological replicates.