Figure 5
From: AtOPR3 specifically inhibits primary root growth in Arabidopsis under phosphate deficiency
The role of GA signaling in regulating primary root growth in Atopr3 and WT plants.
Seedlings were grown as described in Fig. 3. PAC and Ancy were applied to inhibit GA biosynthesis. (a) Effects of 7.5 μM GA (-P + GA), 7.5 μM PAC (-P + PAC), 5 μM Ancy (-P + Ancy) on primary root growth. Results were presented as means (n = 30) with error bars (standard deviation). Asterisks indicated significant differences as determined by a t-test analysis (**P < 0.01). (B-D) Relative expression levels of genes mediating GA biosynthesis (determined by RT-qPCR) in WT and Atopr3 mutants. (b) GA 20-oxidases, (c) GA 3-oxidases and (d) GA 2-oxidases. Data represented means and SD of three independent biological replicates. Different letters indicated means with significant differences (P < 0.05).
