Figure 2: 5G2 mAb promoted C45-2D cell detachment and inhibited cell migration on collagen-coated dishes. | Scientific Reports

Figure 2: 5G2 mAb promoted C45-2D cell detachment and inhibited cell migration on collagen-coated dishes.

From: Generation of a monoclonal antibody recognizing the CEACAM glycan structure and inhibiting adhesion using cancer tissue-originated spheroid as an antigen

Figure 2

(a) Cresyl violet staining of C45-2D cells. Confluent C45-2D cells were incubated with 5G2 mAb for 3 days. Scale bar, 100 μm. (b) Quantification of cell detachment areas when treated with the indicated antibodies. Data indicate mean ± SD from three independent experiments. Differences were analysed by the Kruskal-Wallis test. (c) Quantification of cell migration. C45-2D cell migration was assessed by the percentage of closure after 3 days in culture after removal of the culture insert and antibody addition. Data indicate mean ± SD from four independent experiments. Differences were analysed by the Kruskal-Wallis test. (d) Representative images of the cell migration assay in (c). The antibody doses were 30 μg/ml. The white dotted lines indicate the edge of the space. Scale bar, 100 μm.

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