Figure 4: 5G2 mAb recognized asialo-type N-glycan structure. | Scientific Reports

Figure 4: 5G2 mAb recognized asialo-type N-glycan structure.

From: Generation of a monoclonal antibody recognizing the CEACAM glycan structure and inhibiting adhesion using cancer tissue-originated spheroid as an antigen

Figure 4

(a) Western blot of glycosidase digested membrane fractions of C45 CTOSs using 5G2 mAb. (b) Western blot of lysate from tunicamycin-treated cells using 5G2 mAb. C45 CTOSs and C45-2D cells were cultured with the indicated doses of tunicamycin for 2 days. (c,d) Western blot of sialic acid-digested cell lysate using 5G2 mAb. Protein lysates from CTOSs (c) and cell lines (d) were digested by neuraminidase. Actin is shown as a loading control. (e) Glycan microarray for determination of the glycan structure recognized by 5G2 mAb. The names of glycan structures with high affinity are indicated.

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