Figure 5: CEACAM5 and CEACAM6 were major carriers of 5G2 antigens. | Scientific Reports

Figure 5: CEACAM5 and CEACAM6 were major carriers of 5G2 antigens.

From: Generation of a monoclonal antibody recognizing the CEACAM glycan structure and inhibiting adhesion using cancer tissue-originated spheroid as an antigen

Figure 5

(a) Western blot of lysate from siRNA-transduced C45 CTOSs using the indicated antibodies. Actin is shown as a loading control. (b) Western blot of lysate from T84 cells in which HA-tagged CEACAM5 and CEACAM6 were forcibly expressed. Cell lysates were immunoprecipitated by HA antibody and detected by HA, CECAM5, CEACAM6, or 5G2 antibodies. The two major bands are indicated by arrowheads. (c) Western blot of lysate from colorectal cancer CTOS lines with CEACAM5, CEACAM6, and 5G2 antibodies. Actin is shown as a loading control.

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