Figure 7: Pre-incubation with 5G2 mAb inhibited translocation of integrin β4 in CTOSs. | Scientific Reports

Figure 7: Pre-incubation with 5G2 mAb inhibited translocation of integrin β4 in CTOSs.

From: Generation of a monoclonal antibody recognizing the CEACAM glycan structure and inhibiting adhesion using cancer tissue-originated spheroid as an antigen

Figure 7

(a) Immunocytochemical analysis of CTOSs using integrin β4 antibody. C45 CTOSs under floating conditions (i.e., suspension culture) were pre-incubated with the indicated antibody and cultured for 20 h in Matrigel. (b) CTOS surface coverage with the integrin β4 signal. C45 CTOSs were treated the same as in (a). Differences were analysed by the Mann Whitney U-test. IgG, n = 111; 5G2, n = 139. Horizontal bar, median; boxes, 25th and 75th percentiles; bars, 10th and 90th percentiles. (c) Integrin β4 signal intensity on lateral membranes in CTOSs. C45 CTOSs were treated the same as in (a). Data indicate mean ± SD. Differences were analysed by the Mann Whitney U-test. IgG, n = 1512; 5G2, n = 2066. (d) Western blot using phosphor-FAK (pFAK) or total FAK (tFAK) antibody. C45 CTOSs in floating conditions (F) were pre-incubated with the indicated antibody and cultured for 6 h in Matrigel (G). (e) Immunoprecipitation analysis of floating cultured C45 CTOSs. Antibodies used for immunoprecipitation (IP) and detection (Blot) are shown.

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