Figure 2
From: TCF7L2 involvement in estradiol- and progesterone-modulated islet and hepatic glucose homeostasis
Silencing of TCF7L2 reduces estradiol (E2)- and progesterone (P4)-promoted β-cell insulin/proinsulin secretion.
MIN6 cells were plated at 5 × 105 cells per well in 6-well plates and exposed to a TCF7L2-specific short hairpin RNA (shTCF7L2) or a scrambled shRNA (shScr) for 72 h, then cultured for 24 h in the presence of 100 nM E2 or 1 μM P4. (A) Western blots showing the TCF7L2 protein content after E2 or P4 treatment. (B) Viable cells. (C,D) Basal and stimulated insulin/proinsulin secretions (normalized to viable cell numbers). (E,F) Stimulatory indexes. (G) Proinsulin-to-insulin ratio. *P < 0.05 vs. sex hormone treatment control; #P < 0.05 shTCF7L2 vs. shScr.
