Figure 5
From: TCF7L2 involvement in estradiol- and progesterone-modulated islet and hepatic glucose homeostasis
TCF7L2 involves in estradiol (E2)- and progesterone (P4)-regulated hepatic glucose metabolism.
HepG2 cells (2.5 × 105 cells per well) were seeded in 6-well plates and exposed to a TCF7L2-specific short hairpin RNA (shTCF7L2) or a scrambled shRNA (shScr) for 72 h, or transfected with TCF7L2-IRES2-EGFP (OE-TCF7L2) or a control vector (CV) for 72 h, then cultured for 24 h in the presence of 100 nM E2 or 1 μM P4. (A,B) 2-NBDG uptake. (C,D) Glucose production. (E,F) Western blot. *P < 0.05 vs. sex hormone treatment control; #P < 0.05 shTCF7L2 vs. shScr or OE-TCF7L2 vs. CV.
