Figure 4 | Scientific Reports

Figure 4

From: Hepatitis B virus genome replication triggers toll-like receptor 3-dependent interferon responses in the absence of hepatitis B surface antigen

Figure 4

HBV-induced interferon responses are mediated by F4/80+ cells (KCs) and liver sinusoidal endothelial cells (LSECs).

Primary murine hepatocytes (PMH), KCs, LSECs and a remaining cell fraction were isolated from two-month-old transgenic HBV (HBV-s-mut) mice and from HBV-negative littermates (WT). RNA was extracted; gene expression of Ifnb1 (A), Isg15 (B) and Ifit1 (C) was determined by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Copy numbers were normalized to 100,000 copies of Gapdh (mean ± SEM). Group size n = 4 animals; *p < 0.05, **p < 0.01, ***p < 0.001; ns, not significant.

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