Figure 6
Activation of TLR3 by Poly(I:C) in HBV-s-mut mice is suppressed but still effective in vivo.
Two-month-old male transgenic HBV (HBV-s-mut) mice and their wild type (WT) littermates were given Toll-like receptor 3 (Tlr3) ligand polyinosinic-polycytidylic acid (Poly[I:C]; 4 μg/g body weight) via tail vein injection. Mice were put to death 6 h or 24 h after injection. RNA was extracted and changes in gene expression of Ifnb1 (A), Isg15 (B) and HBV (C) were determined by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Copy numbers were normalized to 100,000 copies of Gapdh (mean ± SEM). DNA was extracted from liver tissue and changes in HBV DNA were quantified by PCR (D). Proteins were extracted from liver tissue, HBcAg was detected by western blot analysis and densitometric analysis was performed (E). Group size n = 3 animals; *p < 0.05, **p < 0.01, ***p < 0.001; HBV-s-mut, Tg1.4HBV-S-Mut3; w/o, without treatment.
