Table 1 Characteristics of HBV replication in HBVs-mut and HBV-s-rec strains.

From: Hepatitis B virus genome replication triggers toll-like receptor 3-dependent interferon responses in the absence of hepatitis B surface antigen

 

HBV-s-mut (n = 4)

HBV-s-rec (n = 4)

p-value

(mean ± SEM)

(mean ± SEM)

HBsAg SERUM [S/CO]

2.0 ± 0.0

226.2 ± 16.2

0.0008

DNA LIVER (copy no./μg DNA)

7,802 ± 2,615

4,722 ± 2,328

0.5176

HBeAg SERUM [S/CO]

56.2 ± 15.6

43.4 ± 9.0

0.5064

HBcAg LIVER (signal intensity HBcAg/Gapdh)

0.84 ± 0.13

1.1 ± 0.1

0.0842

large HBsAg LIVER (signal intensity HBsAg/Gapdh)

3.8 ± 1.1

28.3 ± 3.0

0.0002

middle HBsAg LIVER (signal intensity HBsAg/Gapdh)

3.0 ± 0.8

23.7 ± 1.9

<0.0001

small HBsAg LIVER (signal intensity HBsAg/Gapdh)

3.4 ± 0.5

29.8 ± 1.5

0.0013

De-Ritis-Ratio (AST/ALT)

1.5 ± 0.2

2.8 ± 0.7

0.1025

  1. Footnote: Two-month-old transgenic HBV mice were put to death, liver and serum samples were collected. DNA was extracted from liver tissue and changes in DNA replication of HBV were determined by quantitative PCR (copy no./μg DNA). Hepatitis B excretory antigen (HBeAg) and Hepatitis B surface antigen (HBsAg) levels in the serum were measured by chemiluminescent microparticle immunoassay (CMIA, [S/CO] = sample relative light units (RLU) per cutoff). Proteins were extracted from liver tissue and HBV core (HBcAg) or HBsAg were detected by Western blot analysis. Densitometric analysis was performed. Serum parameter (alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) were determined with the Spotchem™II analyzer. Significant changes are given in bold.
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