Figure 1 | Scientific Reports

Figure 1

From: Protecting enzymatic function through directed packaging into bacterial outer membrane vesicles

Figure 1

PTE packaging and stability schematic.

Pictured above is a schematic representation of bacterial produced free-PTE and isopeptide bonded N-terminal OmpA-SpyTag/PTE-SpyCatcher packaged within an outer membrane vesicle (OMV). The fusion of PTE to the transmembrane porin protein (OmpA) through the SpyTag/SpyCatcher construct facilitates incorporation of the PTE within the OMV. The OMV provides a protective environment that reduces inactivation of the encapsulated enzyme under nearly all storage conditions resulting in prolonged shelf-life and enhanced stability.

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