Figure 2
From: Protecting enzymatic function through directed packaging into bacterial outer membrane vesicles
Long term and varied temperature enzyme stability.
(a) PTE, (b) PTE-SpyCatcher (PTE-SC) and (c) N-terminal OmpA-SpyTag/PTE-SpyCatcher OMVs (PTE/OMVN) were assayed for PTE activity over a variety of storage conditions over the course of 14 days. PTE activity was assayed via initial velocity determination utilizing paraoxon as a substrate monitoring product formation at 405 nm. Percent activity was normalized to an initial PTE activity measurement for each sample (Day 0). Both PTE and PTE-SC demonstrated poor stability across all storage conditions and exhibited heightened sensitivity to inactivation due to freezing and long term storage. OMV packaged PTE maintained 34.5 ± 0.2% activity after 14 days at 37 °C compared to PTE and PTE-SC which did not exhibit any activity under the same conditions. All data represents means (±one standard deviation) of triplicate experiments.
