Figure 4
From: Protecting enzymatic function through directed packaging into bacterial outer membrane vesicles
OMV stability to lyophilization and rehydration.
(a) PTE, PTE-SC and PTE/OMVN were assayed for stability post lyophilization in PBS pH 7.4 with no protectants. Lyophilized samples were stored at RT for 14 days. Non-OMV encapsulated PTE did not survive the lyophilization process compared to a >60% retained activity by OMV encapsulated PTE-SC. (b) OMV size distribution and recovery post freeze-thaw and lyophilization were assessed on a NanoSight LM10 particle tracking system. The OMVs retained both their size distribution and absolute particle number post rehydration. All data represents means (±one standard deviation) of triplicate experiments.
