Figure 5
From: Protecting enzymatic function through directed packaging into bacterial outer membrane vesicles
Proof of concept paraoxon remediation.
Larger-scale remediation of paraoxon utilizing lyophilized PTE/OMVN and PTE/OMVC. (a) Lyophilized OMV powder was added directly to paraoxon contaminated water in scintillation vials and pictures were taken over a 3 h period of time. (b) Paraoxon degradation was monitored at 405 nm demonstrating complete remediation within 90 and 180 min for the PTE/OMVN and PTE/OMVC, respectively. (c) Absorbance spectrums were taken to demonstrate complete conversion of paraoxon (280 nm) to p-nitrophenol (405 nm). Despite differing amounts of initial PTE, complete remediation of paraoxon was observed by both OMV constructs.
