Figure 6
From: Protecting enzymatic function through directed packaging into bacterial outer membrane vesicles
Long-term paraoxon remediation.
After 4 days at RT storage a second remediation cycle utilizing the exact same samples from Fig. 5 were carried out with an equal quantity of paraoxon. (a) Progress curves were monitored for paraoxon degradation at 405 nm with complete remediation utilizing the PTE/OMVN within 180 min. (b) Absorbance spectrums were taken after 4 h to demonstrate complete conversion of paraoxon (280 nm) to p-nitrophenol (405 nm). [PTE/OMVN Pre: spectrum prior to the addition of the second bolus of paraoxon, PTE/OMVN Initial: spectrum directly following the addition of the second bolus of paraoxon, PTE/OMVN Post: spectrum taken at the end of the remediation]. (c) Despite differing amounts of initial PTE, both PTE/OMVN and PTE/OMVC were still capable of complete remediation of the paraoxon with 53 and 47% retained activity, respectively. All data represents means (±one standard deviation) of triplicate experiments.
