Figure 6
From: High activity of the stress promoter contributes to susceptibility to stress in the tree shrew
The active GRE site recruits trans-activators to form complexes for positive modulation of CRH transcription in Tree shrew.
(A) STRING analysis of protein interaction networks of GR and SRC-1 related transcription factors in human and tree shrew. (a,c) Human, (b,d) Tree shrew. (B) ChIP Assay of co-activators associated with aGRE bound GR dimers were performed using antibodies against SRC-1, CRSP150 and Trap220 in tree shrew cortex and hypothalamus brain tissues. (PCR primers targeted at the aGRE sequence) Those factors form complex with GR to bind the aGRE site. (C) Co-IP Assay of GR interaction with co-activators. IP Antibody: GR, IB Antibody: SRC-1, CRSP150 and Trap220. (D)Schematic of GR organized activation complex in initiating CRH transcription.
