Figure 1 | Scientific Reports

Figure 1

From: LIMK Regulates Tumor-Cell Invasion and Matrix Degradation Through Tyrosine Phosphorylation of MT1-MMP

Figure 1

MT1-MMP is a partner and substrate of LIMK1/2.

(A) Schema of the different domains of MT1-MMP and LIMK (B,C) Immunodetection of respectively LIMK1 (B) or LIMK2 (C) with MT1-MMPmCh WT beads trap in MDA-MB-231 cell lysates. Bound proteins were analyzed by immunoblotting with antibodies against MT1-MMP, LIMK1 and LIMK2. Input lysates (1%) were loaded as control. Arrow indicates specific LIMK1 band. (D) Immunodetection of LIMK1 and LIMK2 in anti-MT1-MMP immunoprecipitates in contrast to control mouse IgGs (anti-HA) in MDA-MB-231 cell lysates. Bound proteins were analyzed by immunoblotting with antibodies against MT1-MMP, LIMK1 and LIMK2. Input lysates (1%) were loaded as control. Black arrow indicates LIMK2; red arrow indicates MT1-MMP; blue asterix indicates high chain IgGs. (E) Tyrosine phosphorylation by LIMK1 of MT1-MMPmCh WT (F) Tyrosine phosphorylation by LIMK1 of MT1-MMPmCh WT and not of MT1-MMPmCh ΔCter beads trap. (G) Tyrosine phosphorylation by LIMK1 of MT1-MMPmCh WT beads trap after treatment with DMSO or Pyr1 (20 μM) (H) Tyrosine phosphorylation of MT1-MMPmCh WT after treatment with DMSO or Pyr1 (20 μM).

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