Figure 3 | Scientific Reports

Figure 3

From: LIMK Regulates Tumor-Cell Invasion and Matrix Degradation Through Tyrosine Phosphorylation of MT1-MMP

Figure 3

LIMK1 control cortactin recruitment on MT1-MMP endosomes.

(A) Quantification of cortactin on MT1-MMPmCh-containing endosomes. Y-axis indicates mean cortactin intensity associated with MT1-MMP endosomes normalized to the value in control DMSO-treated cells (in percentage) ± SEM (n is the number of cells analyzed, from at least 3 independent experiments). ***P < 0.001. (B) MDA-MB-231 cells stably expressing MT1-MMPmCh were treated with the indicated siRNAs (50 μM), plated on gelatin and stained for cortactin (in green). Z-projection. Scale bars, 5 μm (entire cell); 1 μm (boxed region at higher magnification). (C) Quantification of cortactin on MT1-MMPmCh vesicles as in panel A. The x-axis indicates mean cortactin intensity associated with MT1-MMPmCh-containing endosomes normalized to the value in control siNt-treated cells (in percentage) ± SEM (n is the number of cells analyzed, from at least 3 independent experiments). ***P < 0.001. (D,E) Overexpression of LIMK1-GFP (D) or LIMK2-GFP (E) in MT1-MMPmCh overexpressing MDA-MB-231 cells. Z-projection. Scale bars, 5 μm (entire cell); 1 μm (boxed region at higher magnification).

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