Figure 3

Sera from mice injected with geOMVs displaying S. pneumoniae serotype 14 capsule antigens cross react with the S. pneumoniae capsule.

(a) Western blots of geOMVs from CLM37 strain with vector control (VC) or S. pneumoniae serotype 14 capsule (CPS14). The S. pneumoniae serotype 14 capsule was visualized by either the commercially available rabbit anti-capsule serotype 14 antibody (red) and the mice sera (green). The overlapping signals are shown in yellow (merged). (b) Pneumococcal geOMVs display the similar immunogenic response as the commercial available Prevnar 13 pneumococcal vaccine. Mice sera (n = 10) collected at day 42 were incubated in wells of ELISA plates seeded with whole cell S. pneumoniae serotype 14. Figure shows statistically significant differences between means for each group. (*p-value < 0.0001; **p-value < 0.0001; NS p-value 0.0298). Error bars are SD and P values were calculated using the unpaired t-test.