Figure 5

geOMVs displaying the N-heptasaccharide reduce C. jejuni colonization in chicken.

(a) Western blots of E. coli EVV11 geOMVs displaying no oligosaccharides (lane 1) and the heptasaccharide N-glycan (lane 2). (b) Campylobacter colonization in chicken. Group 1, non-vaccinated, not challenged (background control); group 2, non-vaccinated, challenged (colonization control); group 3, vaccinated with empty OMVs and challenged; group 4, vaccinated with geOMVs expressing the N-glycan and challenged. Each data point represents cfu/gram cecal content for one bird. The median for each group is represented by a black bar. No significant difference in colonization could be observed between groups 2 and group 3 (NS p-value = 0.29). A statistically significant reduction in colonization was observed in group 4 birds that received the Cj-N-glycan presenting OMVs when compared to either group 3 (*p-value = 0.045) or group 2 (**p-value = 0.032). No Campylobacter cells were detected in group 1, the detection limit based on the experimental setup was 200 cfu/gram cecal content. P-values were calculated by the one-way ANOVA test.