Figure 2: Fisetin reduces the levels of sarkosyl-insoluble tau. | Scientific Reports

Figure 2: Fisetin reduces the levels of sarkosyl-insoluble tau.

From: Fisetin stimulates autophagic degradation of phosphorylated tau via the activation of TFEB and Nrf2 transcription factors

Figure 2

HEK 293 cells were co-transfected with plasmids expressing tau and constitutively active HA-GSK-3β-S9A, and subsequently treated with 10 μM fisetin (FST) for 24 h. RIPA-soluble (RS), sarkosyl-soluble (SS) and sarkosyl-insoluble (SI) fractions from the cells were prepared according to the protocol described in Methods. (a) The levels of tau and HA-GSK-3β-S9A in the cellular fractions were analyzed by immunoblotting using the total tau and HA antibodies, respectively. (b) Bar graphs represent the relative ratio of sarkosyl-insoluble tau to the level of RIPA-soluble tau normalized with that of actin. Data shown are mean ± SE of three independent experiments and were analyzed using Student’s t test. (**p < 0.01).

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