Figure 5: Fisetin increases the levels of TFEB and Nrf2 localized in nuclei. | Scientific Reports

Figure 5: Fisetin increases the levels of TFEB and Nrf2 localized in nuclei.

From: Fisetin stimulates autophagic degradation of phosphorylated tau via the activation of TFEB and Nrf2 transcription factors

Figure 5

Mouse cortical cells (T4) and rat primary cortical neurons were treated with 5 and 10 μM fisetin for 6 h, respectively. To examine the localization of TFEB and Nrf2, cortical cells and neurons fixed with 4% paraformaldehyde were immunostained using the anti-TFEB and Nrf2 antibodies, respectively. Fluorescence signals were observed using an epifluorescence microscope. (a,c) Shows TFEB (a) and Nrf2 (c) immunosignals in control T4 cells not treated, respectively. (b,d) Shows TFEB (b) and Nrf2 (d) immunosignals in T4 cells treated with 5 μM fisetin, respectively. (e,g) Shows TFEB (e) and Nrf2 (g) immunosignals in control neurons not treated, respectively. (f,h) Shows TFEB (f) and Nrf2 (h) immunosignals in neurons treated with 10 μM fisetin, respectively. Arrows indicate TFEB and Nrf2 localized in the nuclei of T4 cells and primary cortical neurons.

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