Figure 5
From: The Vpu-interacting Protein SGTA Regulates Expression of a Non-glycosylated Tetherin Species
The non-glycosylated tetherin species co-immunoprecipitates with SGTA in the presence of WT Vpu.
293T cells were transfected with the vector expressing FLAG-tagged SGTA without or with Vpu (WT or RKR mutant) and HA-tagged tetherin expression vectors. Twenty-four h post-transfection, cells were lysed and immunoblotted with anti-FLAG (top left panel), anti-Vpu (middle left panel), or anti-HA antibodies (lower left panel) or immunoprecipitated with agarose beads conjugated with anti-FLAG antibodies followed by immunoblotting with anti-FLAG (top right panel), anti-Vpu (middle right panel) or anti-HA antibodies (lower right panel) to detect FLAG-tagged SGTA, Vpu and HA-tagged tetherin, respectively. The mobility of molecular mass standards is shown on the right.
