Table 2 Microarray analysis showing differentially regulated hormone pathway and stress related genes in transgenic GhNAC2 expressing Arabidopsis line L-1 compared to Col.
Gene | Nature of Regulation | Status | Fold change |
|---|---|---|---|
Abscisic acid | |||
 AtPYL8 | Positive (ABA receptor) | Up | 2.79 |
 CYP707A3 | Negative (reduction of ABA levels) | Down | 4.48 |
 AHG3 | Negative (encodes protein phosphatase that inhibits SnRKs) | Down | 2.66 |
 AtWRKY18 | Negative | Down | 9.6 |
 AtWRKY40 | Negative | Down | 11.64 |
 AtWRKY70 | Negative regulator of stomatal closure | Down | 3.73 |
 CIPK14 | Negative regulator of ABA responses | Down | 3.0 |
Ethylene | |||
 ACS6 | Positive (Ethylene biosynthesis) | Down | 8.6 |
 MPK3 | Positive (MAPK-activator of stress ethylene) | Down | 2.46 |
 MKK9 | Positive (activator of MAPK3/6) | Down | 2.3 |
 ERF1 | Positive (Ethylene response) | Down | 5.2 |
 ERF2 | Positive (Ethylene response) | Down | 2.74 |
 ERF6 | Positive (Ethylene response) | Down | 5 |
 ERF11 | Ethylene/ABA response | Down | 11.5 |
 EBF2 | Negative (Depletion of EIN3) | Up | 3.1 |
 WRKY33 | Positive (Activator of ERF6) | Down | 4.44 |
Jasmonic acid | |||
 LOX3 | Positive (JA biosynthesis) | Down | 13.6 |
 LOX4 |  | Down | 53.3 |
 AOS |  | Down | 2.29 |
 AOC3 |  | Down | 22 |
 ERD12 |  | Down | 3 |
 CYP94C1 | Negative (JA catabolism) | Down | 11.31 |
 JAZ1 | Negative (JA response inhibitors) | Down | 9.86 |
 JAZ2 |  | Down | 3.3 |
 JAZ5 |  | Down | 19 |
 JAZ6 |  | Down | 3 |
 JAZ7 |  | Down | 50.7 |
 JAZ9 |  | Down | 2.89 |
 MYC2 | Positive (TF that activates JA response) | Down | 6.5 |
Gibberellic acid | |||
 GA2OX2 | Negative (Depletion of active GA) | Down | 2.72 |
 GASA1 | Positive (GA responsive) | Up | 4.11 |
Stress responsive genes (that reduce growth upon over-expression) | |||
 RD26 | Positive (Drought response) | Down | 6.6 |
 NAC3 | Positive (ANAC055; drought responsive) | Down | 4.47 |
 RD29A | Positive (Drought response) | Down | 4.87 |
 MYB44 | Negative regulator of stomatal closure (Activator of WRKY70) | Down | 5.38 |
 ZAT10/STZ | positive (Stress response) | Down | 7.7 |
 ERD9 | positive (Glutathione S Transferase) | Down | 4.1 |
 ERD5 | Positive (Proline dehydrogenase) | Up | 12 |
