Figure 1: Phenotypic confirmation of monocyte-to-osteoclast and monocyte-to-macrophage differentiation.
From: MiR-142-3p is a RANKL-dependent inducer of cell death in osteoclasts
Human CD14+ monocytes were cultured in the presence of M-CSF and sRANKL, or M-CSF only. (A) Representative images of monocytes (day 0), macrophages (day 7) and OC (day 9; TRAP stained). (B) Average number of nuclei per cell for M-CSF + sRANKL (filled bars) and M-CSF only (empty bars) cultures over time. (C) Collagen degradation of M-CSF + sRANKL (filled bar) and M-CSF only (open bar) cultures over time. Data are presented as mean and SD, or representative images, from 4 biological donors. *p < 0.05 relative to monocyte (day 0) controls. P values were calculated using the Student’s t-test.
