Figure 2: miRNA profiling of monocyte to osteoclast differentiation.
From: MiR-142-3p is a RANKL-dependent inducer of cell death in osteoclasts
Human CD14+ monocytes were cultured in the presence of M-CSF+ sRANKL, or M-CSF only, and total RNA was isolated at day 0, 1, 3, 6 & 12. (A) Heat map diagram of hierarchical clustering of samples and relative miRNA expression (top 30) for OC differentiation. (B) Heat map diagram of hierarchical clustering of samples and relative miRNA expression (top 30) for macrophage differentiation. (C) Expression of miR-142-3p over time for OC (left) and macrophage (right) as identified by microarray (main) and RT-PCR (insert). (D) Venn diagram of significantly differentially expressed miRNAs identified during monocyte-to-osteoclast and monocyte-to-macrophage differentiation. (E) Bar chart comparing the total number of upregulated (open bar) and downregulated (filled bar) miRNAs in both groups. Data are presented as mean and SD from 4 biological donors. P values were calculated by ANOVA and adjusted using the Benjamini Hochberg method.
