Figure 3: Increased cell death of osteoclast precursors by overexpression of miR-142-3p.
From: MiR-142-3p is a RANKL-dependent inducer of cell death in osteoclasts
Human CD14+ monocytes were cultured in the presence of M-CSF and sRANKL, or M-CSF only. Cultures were left untreated or transfected with miR-142-3p mimic, miR-142-3p inhibitor, or control mimic at day 2. (A) Brightfield images of differentiating osteoclasts (M-CSF and sRANKL) were taken at day 4 (top) and images of cells stained with Hoescht (nuclei; blue) and MitoTracker (mitochondria, red) were taken at day 9 (bottom). Red lines highlight areas of significant cell-cell contact. (B) Viability of M-CSF plus sRANKL, and M-CSF only, cultures assessed at different time-points by MTS assay, relative to untransfected cells. Filled black squares represent miR-142-3p mimic transfected cells, filled grey squares represent miR-142-3p inhibitor transfected cells, and empty circles represent control mimic transfected cells. Data are presented as mean and SD, or representative images, from 5 biological donors. *p < 0.05 relative to untreated control cells. P values were calculated using the Student’s t-test.
